Composite

Part:BBa_K3282005:Design

Designed by: Mateusz Piotr Szewczyk, Manjyot Kaur, Julia Thomasson, Savvina Leontakianakou   Group: iGEM19_Lund   (2019-10-11)


pbrD and pbrT under T7 promoter


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 1444
    Illegal NheI site found at 1642
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal NgoMIV site found at 1392
    Illegal NgoMIV site found at 1855
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal SapI site found at 1999
    Illegal SapI.rc site found at 2229


Design Notes

Used a T7 promoter to express the proteins in E. coli BL21-DE3.


Source

The coding sequence is based on the lead binding protein K3282002 and lead transport protein K3282001. The T7 promoter I719005 and Anderson ribosome binding site J61109 are used to express the proteins, followed by a TE terminators B0012.

References